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deedo View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote deedo Quote  Post ReplyReply Direct Link To This Post Posted: March 19 2005 at 6:43am

I was thinking about the dry wieght vs. N/P concentrations issue. Thought it'd be fun to bounce some ideas off of the many experienced algae culturists on the board. Seems to me that these algae are all basically plants right. So they're all likely to be mostly made (structurally at least) of cellulose (a polysaccaride.) Some species have multi-nucleated cells (big ol' long tubes of goo) and others have 'true' cells like land loving plants. Can we hypothesize that species with multi-nucleated cells will have relatively less cellulose and more cytoplam? That's the stuff with all the proteins (N and P) and nucleotides (P) after all. No space in between plant cells except for more cellulose. Could we test by growing algae in the presence of a known concentration of phosphate and/or nitrate fertilizers and measuring the reduction of this concentratoin over time? Then we measure the change in wet-weight and the final dry weight and concentration of N and P of the plant matter. We could calculate the ratio nutrient uptake to biomass growth and compare these (linnear?) curves between species. My hypothesis predicts that multinucleated species will have slightly less linnear curves, that is, they'll take up nutrients faster as they gain biomass because their cytoplasm to cellulose ratio inceases faster. A built in controll is that reduction in nitrate and phosphate concentration in the water should be the same as the increase in the plant.

Up waaay too late working. Using this board as a brief diversion. Got very carried away just now. The experiment would take learning quantitative chemistry techniques (which are no fun) and having a bunch of tanks with lights set up (ten maybe). The tanks will probably have to be heated and will definately need to be isolated from each other. Seemslike an ovwervwelmingly crapload of work now that I think of it. Plus, I live in a two betwoom apartment and wouldn't want to be up to the eyeballs in algae. Maybe I just better begin sleeing now.

g'night y'all



Edited by deedo
"Science is like a blabbermouth who ruins the movie by telling you how it ends. Well, I say there are some things we don't want to know. Important things!" - Ned Flanders
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Adam Blundell View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote Adam Blundell Quote  Post ReplyReply Direct Link To This Post Posted: March 19 2005 at 8:32am

Can we hypothesize that species with multi-nucleated cells will have relatively less cellulose and more cytoplam?

I'm not sure. 

Could we test by growing algae in the presence of a known concentration of phosphate and/or nitrate fertilizers and measuring the reduction of this concentratoin over time?

Fortunately this has been done, so we don't need to.  The most interesting findings from that study were that different aglae (from the same Genus even!) had great variation in the amount of N to P assimilated.

Adam

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Mark Peterson View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote Mark Peterson Quote  Post ReplyReply Direct Link To This Post Posted: March 19 2005 at 9:15am
Isn't this variation between individuals within a species the very thing that Darwin and subsequent researchers started to discover and then postulate that there were other factors determining the outcome of offspring characteristics which led to the discovery of DNA, etc. and the current field of Genetics? Wow, what a run on sentence!

Anyway, we are always saying that each fish or coral behaves differently in each tank. All Yellow Tangs are not alike, that they have individual personality... Wow. This sure makes life fascinating...
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FISHMAN Dan View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote FISHMAN Dan Quote  Post ReplyReply Direct Link To This Post Posted: March 26 2005 at 5:35am

Instead of testing dry weight, why not test water samples in separate containers each dosed with a uniform amount of nitrate and phosphate and lit with the same amount of light and which include the same amount of different species of algae.

Iam not convinced this dry weight is of any scientific value, what should be measured is how much nutrients can be removed from water in a given amount of time by a particular species.

125 reef, grower of hard to find macro algea,(feather giant feather, branching coraline ect...
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Post Options Post Options   Thanks (0) Thanks(0)   Quote FISHMAN Dan Quote  Post ReplyReply Direct Link To This Post Posted: March 26 2005 at 5:48am

I would suggest having say 4 5gal tanks 1 would be a control and the other four would be filled with equal weights of growing tips of various algea.

then the tanks would be filled with water known to contain a certain amount of both phos and nitrates iron and whatever else to be tested.

All tanks would be lit, heated  and areated equally.

concentrations of the nutrients would be measured daily, and the winner determined not by mere dry weight but by the amount of nutrients removed from the water itself.

After all  the point is water quality not plant weight.

125 reef, grower of hard to find macro algea,(feather giant feather, branching coraline ect...
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Adam Blundell View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote Adam Blundell Quote  Post ReplyReply Direct Link To This Post Posted: March 26 2005 at 9:48am

the winner determined not by mere dry weight but by the amount of nutrients removed from the water itself

What's the difference???

Adam

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FISHMAN Dan View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote FISHMAN Dan Quote  Post ReplyReply Direct Link To This Post Posted: March 26 2005 at 4:32pm
well some of the nutrients are used by the algea right? not just stored in the tissue, also it just seems more realistic to test the affect on the water quality itself.
125 reef, grower of hard to find macro algea,(feather giant feather, branching coraline ect...
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Adam Blundell View Drop Down
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Post Options Post Options   Thanks (0) Thanks(0)   Quote Adam Blundell Quote  Post ReplyReply Direct Link To This Post Posted: March 26 2005 at 4:43pm

Dan-
It is a great idea, but it really won't work.  I've never tried, I've just read many reports on people who had that idea and failed.  Basically, you can't grow algae in a sterile tank by adding nitrate and phosphate.  It really needs more "food"... but you can do this experiment by using natural seawater.  That study has been done a few times, then adding different amounts of nitrate and phosphate to see what species of algae remove these two item and in what proportion. 

But far more frequent those researchers end up turning to dry weight as a better gauge for nutrient removal.  But, I certainly encourage you to try the experiment, because if you could do it- it would be great information to have.

Adam

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